Artuvetrin® Skin Test

The Artuvetrin® Skin Test determines to which substances a dog is allergic, by injecting small amounts of different allergens in the pet’s skin (intradermal). Subsequently, it is possible to evaluate whether or not a local reaction develops at the site of the injection.

Based on the clinical history of the patient, it is possible to perform a skin test with the most relevant allergens. We have about 80 different allergens available for skin testing.

  • 80 individual allergens or mixtures available
  • 3 ml vials (enough for 60 tests)
  • Shelf life of 6 months

Download Artuvetrin Skin Test Order Form

Download Package Leaflet for Artuvetrin Skin Test

Before testing

Before carrying out the skin test, any medication that could affect the allergy and itching – including prednisone, cyclosporine and antihistamine – must be suspended for typically 2 – 6 weeks. The skin test is carried out on the lateral thorax of the patient after carefully shaving a patch.

Performing the test

A very small quantity (0.05 ml) of the allergen and control solutions (positive and negative control) is injected into the skin at different places. After 15-20 minutes, all the injection sites are assessed on skin swelling (wheal formation). If a pet is allergic to a certain substance, an inflammation reaction will appear in the skin, expressed in swelling. The extent of swelling is compared with the extent of swelling of the positive and negative control. This way it is possible to determine the substances to which the pet is allergic. Naturally, an animal may be allergic to many substances.

  1. Carefully shave the dog’s lateral thorax wall. The test must be administered on intact skin.
  2. Number the injection sites on the dog’s skin with a marker or pen at intervals of 2.5 cm.
  3. Fill the syringes with the different allergen and control solutions.
  4. Administer 0.05 ml of each allergen and control solution intracutaneously (a bubble will appear after injection) with the number on the holder corresponding to the number written on the skin.
  5. Read the skin reaction after 15-20 minutes by labelling the increase in the wheal with a marker or pen.

Artuvetrin® Skin Test set

Because we have over 80 individual allergens and mixtures available for skin testing, you can compose your own skin test set. You can also use our suggested basic skin test set:

  1. Negative control
  2. Positive control
  3. Grass pollen mix
  4. Tree pollen mix 1
  5. Tree pollen mix 2
  6. Weed pollen mix
  7. Copra mite
  8. Farinae mite
  9. Hay mite
  10. House dust mite
  11. Grain mite
  12. Cat epithelium
  13. Fungal mix
  14. Flea

Interpretation of the test

The reactions are measured after 15 minutes but no later than 20 minutes, and the increased wheal is marked with the skin marker or a highlight marker. In order to evaluate the results you can use a ruler.

The response to the negative control is generally 0 mm.

The allergen with a wheal diameter of more than half the wheal caused by the positive control (or half the diameter of the ‘positive’ wheal minus that of the ‘negative’ wheal) is to be considered as positive.

When evaluating positive reactions, the size and configuration of the wheals are important, but it is just as essential to check whether or not the concerning allergen is relevant.

Also when interpreting positive reactions, it is important to realize that indirect contact (for example, clothing after horse-riding lessons) may lead to relevant, specific reactions.